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Assay Detail

CHEMBL1109007

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of PDE4 expressed in HEK293 cells coexpressing cyclic nucleotide gated ion channel mutant assessed as inhibition of NECA-induced cAMP production treated 15 mins before NECA challenge measured after 45 mins by FLIPR assay
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type HEK293
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Water-soluble PDE4 inhibitors for the treatment of dry eye.
Govek SP, Oshiro G, Anzola JV, Beauregard C, Chen J, Coyle AR, Gamache DA, Hellberg MR, Hsien JN, Lerch JM, Liao JC, Malecha JW, Staszewski LM, Thomas DJ, Yanni JM, Noble SA, Shiau AK.
Bioorg Med Chem Lett (2010) 20 : 2928 -2932
PubMed 20378348 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 6.64 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL193240 ROFLUMILAST 4.0 1 9.00
CHEMBL42126 PICLAMILAST 2.0 1 7.89
CHEMBL1096812 1 7.55
CHEMBL1096813 1 7.52
CHEMBL1098844 1 7.52
CHEMBL1095210 1 7.40
CHEMBL1099322 1 6.92
CHEMBL1099164 1 6.82
CHEMBL1099163 1 6.82
CHEMBL1095865 1 6.75
CHEMBL1098818 1 6.55
CHEMBL1095226 1 6.18
CHEMBL1098542 1 6.14
CHEMBL1098541 1 6.05
CHEMBL70903 1 5.96
CHEMBL1095225 1 5.74
CHEMBL1096814 1 5.27
CHEMBL1098778 1 5.09
CHEMBL1098193 1 5.00
CHEMBL1098503 1 -
CHEMBL1098502 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL193240 ROFLUMILAST IC50 = 1.0 nM 9.00
CHEMBL42126 PICLAMILAST IC50 = 13.0 nM 7.89
CHEMBL1096812 IC50 = 28.0 nM 7.55
CHEMBL1096813 IC50 = 30.0 nM 7.52
CHEMBL1098844 IC50 = 30.0 nM 7.52
CHEMBL1095210 IC50 = 40.0 nM 7.40
CHEMBL1099322 IC50 = 120.0 nM 6.92
CHEMBL1099164 IC50 = 150.0 nM 6.82
CHEMBL1099163 IC50 = 150.0 nM 6.82
CHEMBL1095865 IC50 = 180.0 nM 6.75
CHEMBL1098818 IC50 = 280.0 nM 6.55
CHEMBL1095226 IC50 = 660.0 nM 6.18
CHEMBL1098542 IC50 = 730.0 nM 6.14
CHEMBL1098541 IC50 = 890.0 nM 6.05
CHEMBL70903 IC50 = 1100.0 nM 5.96
CHEMBL1095225 IC50 = 1820.0 nM 5.74
CHEMBL1096814 IC50 = 5350.0 nM 5.27
CHEMBL1098778 IC50 = 8200.0 nM 5.09
CHEMBL1098193 IC50 = 10000.0 nM 5.00
CHEMBL1098503 IC50 > 30000.0 nM -
CHEMBL1098502 IC50 > 30000.0 nM -