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Assay Detail

CHEMBL1109181

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of NFkappa p50 isolated from nuclear extract of human HeLa cells assessed as blockade of binding to biotinylated consesus sequence by chemiluminescence assay
15
Total Activities
15
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HeLa
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Nuclear factor NF-kappa-B p105 subunit (CHEMBL3251)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Cytotoxic and NF-kappaB inhibitory constituents of Artocarpus rigida.
Ren Y, Kardono LB, Riswan S, Chai H, Farnsworth NR, Soejarto DD, Carcache de Blanco EJ, Kinghorn AD.
J Nat Prod (2010) 73 : 949 -955
PubMed 20384315 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 6.38 7.12
Activity 5 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL438139 ROCAGLAMIDE 1 7.12
CHEMBL1099162 ARTOBILOXANTHONE 1 5.64
CHEMBL109860 1 -
CHEMBL516519 CYCLOARTOBILOXANTHONE 1 -
CHEMBL1096733 ARTONIN O 1 -
CHEMBL1096732 ARTORISTILBENE 1 -
CHEMBL1096731 CYCLORIGIDOL 1 -
CHEMBL1096730 ARTORIGIDIN C 1 -
CHEMBL1094757 ARTORIGIDIN B 1 -
CHEMBL1094756 ARTORIGIDIN A 1 -
CHEMBL269277 BETULINIC ACID 1.0 1 -
CHEMBL169 URSOLIC ACID 2.0 1 -
CHEMBL1097723 ARTONIN G 1 -
CHEMBL1096045 ARTONIN K 1 -
CHEMBL1094755 ARTONIN N 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL438139 ROCAGLAMIDE IC50 = 75.0 nM 7.12
CHEMBL1099162 ARTOBILOXANTHONE IC50 = 2300.0 nM 5.64
CHEMBL109860 IC50 > 10000.0 nM -
CHEMBL516519 CYCLOARTOBILOXANTHONE IC50 > 10000.0 nM -
CHEMBL1096733 ARTONIN O IC50 > 10000.0 nM -
CHEMBL1096732 ARTORISTILBENE IC50 > 10000.0 nM -
CHEMBL1096731 CYCLORIGIDOL IC50 > 10000.0 nM -
CHEMBL1096730 ARTORIGIDIN C IC50 - -
CHEMBL1094757 ARTORIGIDIN B IC50 - -
CHEMBL1094756 ARTORIGIDIN A IC50 > 10000.0 nM -
CHEMBL269277 BETULINIC ACID Activity - -
CHEMBL169 URSOLIC ACID Activity - -
CHEMBL1097723 ARTONIN G Activity - -
CHEMBL1096045 ARTONIN K Activity - -
CHEMBL1094755 ARTONIN N Activity - -