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Assay Detail

CHEMBL1109707

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant mTOR assessed as reduction in GFP-4EBP1 phosphorylation after 30 mins by FRET assay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase mTOR (CHEMBL2842)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of GNE-477, a potent and efficacious dual PI3K/mTOR inhibitor.
Heffron TP, Berry M, Castanedo G, Chang C, Chuckowree I, Dotson J, Folkes A, Gunzner J, Lesnick JD, Lewis C, Mathieu S, Nonomiya J, Olivero A, Pang J, Peterson D, Salphati L, Sampath D, Sideris S, Sutherlin DP, Tsui V, Wan NC, Wang S, Wong S, Zhu BY.
Bioorg Med Chem Lett (2010) 20 : 2408 -2411
PubMed 20346656 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 12 7.67 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1091977 1 8.40
CHEMBL1093267 1 8.40
CHEMBL1090601 1 8.30
CHEMBL1090598 1 8.00
CHEMBL1090239 1 8.00
CHEMBL1091978 1 7.68
CHEMBL1083192 1 7.54
CHEMBL1091979 1 7.52
CHEMBL594844 1 7.38
CHEMBL1090599 1 7.30
CHEMBL1090600 1 7.23
CHEMBL521851 PICTILISIB 2.0 1 6.24

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1091977 Ki = 4.0 nM 8.40
CHEMBL1093267 Ki = 4.0 nM 8.40
CHEMBL1090601 Ki = 5.0 nM 8.30
CHEMBL1090598 Ki = 10.0 nM 8.00
CHEMBL1090239 Ki = 10.0 nM 8.00
CHEMBL1091978 Ki = 21.0 nM 7.68
CHEMBL1083192 Ki = 29.0 nM 7.54
CHEMBL1091979 Ki = 30.0 nM 7.52
CHEMBL594844 Ki = 42.0 nM 7.38
CHEMBL1090599 Ki = 50.0 nM 7.30
CHEMBL1090600 Ki = 59.0 nM 7.23
CHEMBL521851 PICTILISIB Ki = 570.0 nM 6.24