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Assay Detail

CHEMBL1111365

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]Flumazenil from cloned human GABA alpha-1-beta-3-gamma-2 receptor expressed in HEK293 cells
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

GABA-A receptor; alpha-1/beta-3/gamma-2 (CHEMBL2094121)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

The discovery and unique pharmacological profile of RO4938581 and RO4882224 as potent and selective GABAA alpha5 inverse agonists for the treatment of cognitive dysfunction.
Knust H, Achermann G, Ballard T, Buettelmann B, Gasser R, Fischer H, Hernandez MC, Knoflach F, Koblet A, Stadler H, Thomas AW, Trube G, Waldmeier P.
Bioorg Med Chem Lett (2009) 19 : 5940 -5944
PubMed 19762240 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 6 7.33 7.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1077391 1 7.82
CHEMBL1077381 1 7.75
CHEMBL1078985 1 7.48
CHEMBL1080412 1 7.28
CHEMBL1078580 1 6.87
CHEMBL1080588 1 6.76

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1077391 Ki = 15.0 nM 7.82
CHEMBL1077381 Ki = 18.0 nM 7.75
CHEMBL1078985 Ki = 33.0 nM 7.48
CHEMBL1080412 Ki = 53.0 nM 7.28
CHEMBL1078580 Ki = 136.0 nM 6.87
CHEMBL1080588 Ki = 174.0 nM 6.76