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Assay Detail

CHEMBL1120675

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]epibatidine from rat alpha4beta2 nAChR
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Neuronal acetylcholine receptor; alpha4/beta2 (CHEMBL1907596)
Type PROTEIN COMPLEX
Organism Rattus norvegicus

Publication

In silico characterization of cytisinoids docked into an acetylcholine binding protein.
Abin-Carriquiry JA, Zunini MP, Cassels BK, Wonnacott S, Dajas F.
Bioorg Med Chem Lett (2010) 20 : 3683 -3687
PubMed 20493692 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 7.57 9.61

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL187394 1 9.61
CHEMBL365956 1 9.51
CHEMBL497939 CYTISINICLINE 3.0 1 7.16
CHEMBL1083905 1 6.99
CHEMBL1085045 1 6.95
CHEMBL1084011 1 6.43
CHEMBL1084012 1 6.34

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL187394 IC50 = 0.2455 nM 9.61
CHEMBL365956 IC50 = 0.309 nM 9.51
CHEMBL497939 CYTISINICLINE IC50 = 69.18 nM 7.16
CHEMBL1083905 IC50 = 102.33 nM 6.99
CHEMBL1085045 IC50 = 112.2 nM 6.95
CHEMBL1084011 IC50 = 371.54 nM 6.43
CHEMBL1084012 IC50 = 457.09 nM 6.34