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Assay Detail

CHEMBL1167866

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human SGLT2 expressed in HEK293 cells assessed as inhibition of [14C]alpha-methyl-D-glucopyranoside uptake after 1.5 hrs by liquid scintillation counting
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Sodium/glucose cotransporter 2 (CHEMBL3884)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

ortho-Substituted C-aryl glucosides as highly potent and selective renal sodium-dependent glucose co-transporter 2 (SGLT2) inhibitors.
Xu B, Feng Y, Lv B, Xu G, Zhang L, Du J, Peng K, Xu M, Dong J, Zhang W, Zhang T, Zhu L, Ding H, Sheng Z, Welihinda A, Seed B, Chen Y.
Bioorg Med Chem (2010) 18 : 4422 -4432
PubMed 20576578 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 8.19 9.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1164052 1 9.52
CHEMBL1165446 1 9.05
CHEMBL1163912 1 9.05
CHEMBL1165840 1 8.92
CHEMBL1163590 1 8.80
CHEMBL496138 1 8.72
CHEMBL1163593 1 8.52
CHEMBL1163532 1 8.44
CHEMBL1163754 1 8.22
CHEMBL565784 1 8.19
CHEMBL1163914 1 8.17
CHEMBL429910 DAPAGLIFLOZIN 4.0 1 8.17
CHEMBL1165643 1 7.92
CHEMBL1163823 1 7.80
CHEMBL1164445 1 7.28
CHEMBL1163753 1 6.96
CHEMBL1163692 1 6.89
CHEMBL1163592 1 6.77
CHEMBL1163687 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1164052 IC50 = 0.3 nM 9.52
CHEMBL1165446 IC50 = 0.9 nM 9.05
CHEMBL1163912 IC50 = 0.9 nM 9.05
CHEMBL1165840 IC50 = 1.2 nM 8.92
CHEMBL1163590 IC50 = 1.6 nM 8.80
CHEMBL496138 IC50 = 1.9 nM 8.72
CHEMBL1163593 IC50 = 3.0 nM 8.52
CHEMBL1163532 IC50 = 3.6 nM 8.44
CHEMBL1163754 IC50 = 6.0 nM 8.22
CHEMBL565784 IC50 = 6.5 nM 8.19
CHEMBL1163914 IC50 = 6.7 nM 8.17
CHEMBL429910 DAPAGLIFLOZIN IC50 = 6.7 nM 8.17
CHEMBL1165643 IC50 = 12.0 nM 7.92
CHEMBL1163823 IC50 = 16.0 nM 7.80
CHEMBL1164445 IC50 = 52.0 nM 7.28
CHEMBL1163753 IC50 = 110.0 nM 6.96
CHEMBL1163692 IC50 = 130.0 nM 6.89
CHEMBL1163592 IC50 = 170.0 nM 6.77
CHEMBL1163687 IC50 < 10.0 nM -