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Assay Detail

CHEMBL1176402

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]methylspiperone from human cloned 5HT2A receptor expressed in HEK293 cells after 90 mins by liquid scintillation counting
34
Total Activities
34
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

5-hydroxytryptamine receptor 2A (CHEMBL224)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and structure-affinity relationships of novel small molecule natural product derivatives capable of discriminating between serotonin 5-HT1A, 5-HT2A, 5-HT2C receptor subtypes.
Cummings DF, Canseco DC, Sheth P, Johnson JE, Schetz JA.
Bioorg Med Chem (2010) 18 : 4783 -4792
PubMed 20570529 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 34 6.42 7.20

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL43916 1 7.20
CHEMBL296090 1 7.20
CHEMBL40726 1 7.00
CHEMBL43929 1 7.00
CHEMBL42463 1 6.90
CHEMBL96883 1 6.80
CHEMBL276673 1 6.80
CHEMBL42024 1 6.80
CHEMBL1172812 1 6.76
CHEMBL328844 1 6.70
CHEMBL1173820 1 6.63
CHEMBL297043 1 6.60
CHEMBL92656 1 6.60
CHEMBL301784 1 6.60
CHEMBL299726 1 6.50
CHEMBL44095 1 6.40
CHEMBL299530 1 6.30
CHEMBL404232 APLYSINOPSIN 1 6.22
CHEMBL289063 1 6.20
CHEMBL1173408 1 6.18
CHEMBL38911 1 6.10
CHEMBL93850 1 6.10
CHEMBL433060 1 5.80
CHEMBL42276 1 5.60
CHEMBL290395 1 5.60
CHEMBL54769 1 5.40
CHEMBL41347 1 5.30
CHEMBL59097 1 -
CHEMBL55958 1 -
CHEMBL56232 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL43916 Ki = 63.0 nM 7.20
CHEMBL296090 Ki = 63.0 nM 7.20
CHEMBL40726 Ki = 100.0 nM 7.00
CHEMBL43929 Ki = 100.0 nM 7.00
CHEMBL42463 Ki = 126.0 nM 6.90
CHEMBL96883 Ki = 159.0 nM 6.80
CHEMBL276673 Ki = 159.0 nM 6.80
CHEMBL42024 Ki = 159.0 nM 6.80
CHEMBL1172812 Ki = 173.0 nM 6.76
CHEMBL328844 Ki = 200.0 nM 6.70
CHEMBL1173820 Ki = 235.0 nM 6.63
CHEMBL297043 Ki = 251.0 nM 6.60
CHEMBL92656 Ki = 251.0 nM 6.60
CHEMBL301784 Ki = 251.0 nM 6.60
CHEMBL299726 Ki = 316.0 nM 6.50
CHEMBL44095 Ki = 398.0 nM 6.40
CHEMBL299530 Ki = 501.0 nM 6.30
CHEMBL404232 APLYSINOPSIN Ki = 598.0 nM 6.22
CHEMBL289063 Ki = 631.0 nM 6.20
CHEMBL1173408 Ki = 655.0 nM 6.18
CHEMBL38911 Ki = 794.0 nM 6.10
CHEMBL93850 Ki = 794.0 nM 6.10
CHEMBL433060 Ki = 1585.0 nM 5.80
CHEMBL42276 Ki = 2512.0 nM 5.60
CHEMBL290395 Ki = 2512.0 nM 5.60
CHEMBL54769 Ki = 3981.0 nM 5.40
CHEMBL41347 Ki = 5012.0 nM 5.30
CHEMBL59097 Ki > 10000.0 nM -
CHEMBL55958 Ki > 10000.0 nM -
CHEMBL56232 Ki > 10000.0 nM -
CHEMBL42327 Ki > 10000.0 nM -
CHEMBL1173334 Ki - -
CHEMBL463249 Ki - -
CHEMBL1173333 Ki - -