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Assay Detail

CHEMBL1176449

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human Gli1 expressed in mouse C3H10T1/2 cells assessed as inhibition of transcriptional activity after 24 hrs by luciferase reporter gene assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type C3H 10T1/2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Zinc finger protein GLI1 (CHEMBL5461)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Amide conjugates of ketoprofen and indole as inhibitors of Gli1-mediated transcription in the Hedgehog pathway.
Mahindroo N, Connelly MC, Punchihewa C, Yang L, Yan B, Fujii N.
Bioorg Med Chem (2010) 18 : 4801 -4811
PubMed 20605720 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 5.69 5.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1172835 1 5.80
CHEMBL1173148 1 5.58

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1172835 IC50 = 1600.0 nM 5.80
CHEMBL1173148 IC50 = 2600.0 nM 5.58