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Assay Detail

CHEMBL1220002

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of mouse recombinant N-terminal His6-tagged Abl by radiometric kinase assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase ABL1 (CHEMBL3099)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

The design, synthesis, and evaluation of 8 hybrid DFG-out allosteric kinase inhibitors: a structural analysis of the binding interactions of Gleevec, Nexavar, and BIRB-796.
Dietrich J, Hulme C, Hurley LH.
Bioorg Med Chem (2010) 18 : 5738 -5748
PubMed 20621496 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 6.56 8.07

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1213973 1 8.07
CHEMBL941 IMATINIB 4.0 1 7.97
CHEMBL1213974 1 7.20
CHEMBL1336 SORAFENIB 4.0 1 6.65
CHEMBL1213976 1 6.61
CHEMBL1213975 1 6.24
CHEMBL1213925 1 4.89
CHEMBL103667 DORAMAPIMOD 2.0 1 4.84
CHEMBL1213922 1 -
CHEMBL1213923 1 -
CHEMBL1213924 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1213973 IC50 = 8.6 nM 8.07
CHEMBL941 IMATINIB IC50 = 10.8 nM 7.97
CHEMBL1213974 IC50 = 62.6 nM 7.20
CHEMBL1336 SORAFENIB IC50 = 225.9 nM 6.65
CHEMBL1213976 IC50 = 244.0 nM 6.61
CHEMBL1213975 IC50 = 572.4 nM 6.24
CHEMBL1213925 IC50 = 12930.0 nM 4.89
CHEMBL103667 DORAMAPIMOD IC50 = 14600.0 nM 4.84
CHEMBL1213922 IC50 > 30000.0 nM -
CHEMBL1213923 IC50 > 30000.0 nM -
CHEMBL1213924 IC50 < 1.0 nM -