Assay Detail
Binding
CHEMBL1220212
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of Bacteroides thetaiotaomicron beta-mannosidase 2A N461A mutant expressed in Escherichia coli assessed as inhibition of 4-methyllumbelliferone release by spectrophotometry
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Bacteroides thetaiotaomicron |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Target
Beta-mannosidase (CHEMBL4296293) ↗| Type | SINGLE PROTEIN |
| Organism | Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC10582 / E50 / VPI-5482) |
Publication
Structural and biochemical evidence for a boat-like transition state in beta-mannosidases.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 8 | 4.88 | 5.50 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1213398 | — | — | 1 | 5.50 |
| CHEMBL1213399 | — | — | 1 | 5.14 |
| CHEMBL1213402 | — | — | 1 | 4.88 |
| CHEMBL1213400 | — | — | 1 | 4.72 |
| CHEMBL1213397 | MANNOIMIDAZOLE | — | 1 | 4.55 |
| CHEMBL1213440 | — | — | 1 | 4.46 |
| CHEMBL1213442 | — | — | 1 | - |
| CHEMBL1213444 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1213398 | — | Ki | = | 3186.0 | nM | 5.50 |
| CHEMBL1213399 | — | Ki | = | 7281.0 | nM | 5.14 |
| CHEMBL1213402 | — | Ki | = | 13180.0 | nM | 4.88 |
| CHEMBL1213400 | — | Ki | = | 19130.0 | nM | 4.72 |
| CHEMBL1213397 | MANNOIMIDAZOLE | Ki | = | 28110.0 | nM | 4.55 |
| CHEMBL1213440 | — | Ki | = | 34760.0 | nM | 4.46 |
| CHEMBL1213442 | — | Ki | = | 3750000.0 | nM | - |
| CHEMBL1213444 | — | Ki | = | 641400.0 | nM | - |