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Assay Detail

CHEMBL1221024

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of wild type Bacteroides thetaiotaomicron beta-mannosidase 2A expressed in Escherichia coli assessed as inhibition of 4-methyllumbelliferone release by spectrophotometry
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Bacteroides thetaiotaomicron
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Structural and biochemical evidence for a boat-like transition state in beta-mannosidases.
Tailford LE, Offen WA, Smith NL, Dumon C, Morland C, Gratien J, Heck MP, Stick RV, Blériot Y, Vasella A, Gilbert HJ, Davies GJ.
Nat Chem Biol (2008) 4 : 306 -312
PubMed 18408714 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 8 6.00 7.32

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1213398 1 7.32
CHEMBL1213399 1 7.14
CHEMBL1213400 1 6.29
CHEMBL1213397 MANNOIMIDAZOLE 1 5.77
CHEMBL1213402 1 5.76
CHEMBL1213444 1 5.23
CHEMBL1213440 1 4.48
CHEMBL1213442 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1213398 Ki = 48.0 nM 7.32
CHEMBL1213399 Ki = 73.0 nM 7.14
CHEMBL1213400 Ki = 514.0 nM 6.29
CHEMBL1213397 MANNOIMIDAZOLE Ki = 1700.0 nM 5.77
CHEMBL1213402 Ki = 1752.0 nM 5.76
CHEMBL1213444 Ki = 5836.0 nM 5.23
CHEMBL1213440 Ki = 33230.0 nM 4.48
CHEMBL1213442 Ki = 197100.0 nM -