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Assay Detail

CHEMBL1227291

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Escherichia coli K-12 recombinant His-tagged folylpoly-gamma-glutamate synthetase expressed in Escherichia coli BL21 LC-MS/MS method
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Escherichia coli K-12
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Dihydrofolate synthase/folylpolyglutamate synthase (CHEMBL3309008)
Type SINGLE PROTEIN
Organism Escherichia coli (strain K12)

Publication

A domino effect in antifolate drug action in Escherichia coli.
Kwon YK, Lu W, Melamud E, Khanam N, Bognar A, Rabinowitz JD.
Nat Chem Biol (2008) 4 : 602 -608
PubMed 18724364 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 1 5.51 5.51

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL46294 DIHYDROFOLIC ACID 1 5.51

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL46294 DIHYDROFOLIC ACID Ki = 3100.0 nM 5.51