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Assay Detail

CHEMBL1227415

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of LFA1-mediated Staphylococcal enterotoxin B-stimulated human T cell activation in presence of 10% fetal bovine serum
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type T-cell
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Integrin alpha-L (CHEMBL1803)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of tetrahydroisoquinoline (THIQ) derivatives as potent and orally bioavailable LFA-1/ICAM-1 antagonists.
Zhong M, Shen W, Barr KJ, Arbitrario JP, Arkin MR, Bui M, Chen T, Cunningham BC, Evanchik MJ, Hanan EJ, Hoch U, Huen K, Hyde J, Kumer JL, Lac T, Lawrence CE, Martell JR, Oslob JD, Paulvannan K, Prabhu S, Silverman JA, Wright J, Yu CH, Zhu J, Flanagan WM.
Bioorg Med Chem Lett (2010) 20 : 5269 -5273
PubMed 20655213 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 6.26 7.16

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1222290 1 7.16
CHEMBL1222021 1 6.62
CHEMBL1222288 1 6.58
CHEMBL1222022 1 6.37
CHEMBL1222132 1 6.26
CHEMBL1222289 1 6.19
CHEMBL1221969 1 5.92
CHEMBL1221972 1 5.80
CHEMBL1222133 1 5.42
CHEMBL1222291 1 -
CHEMBL1221919 1 -
CHEMBL1221970 1 -
CHEMBL1221971 1 -
CHEMBL1222229 1 -
CHEMBL1222228 1 -
CHEMBL1222227 1 -
CHEMBL1222226 1 -
CHEMBL1222131 1 -
CHEMBL1222024 1 -
CHEMBL1222023 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1222290 IC50 = 69.0 nM 7.16
CHEMBL1222021 IC50 = 240.0 nM 6.62
CHEMBL1222288 IC50 = 260.0 nM 6.58
CHEMBL1222022 IC50 = 430.0 nM 6.37
CHEMBL1222132 IC50 = 550.0 nM 6.26
CHEMBL1222289 IC50 = 650.0 nM 6.19
CHEMBL1221969 IC50 = 1200.0 nM 5.92
CHEMBL1221972 IC50 = 1600.0 nM 5.80
CHEMBL1222133 IC50 = 3800.0 nM 5.42
CHEMBL1222291 IC50 - -
CHEMBL1221919 IC50 - -
CHEMBL1221970 IC50 - -
CHEMBL1221971 IC50 - -
CHEMBL1222229 IC50 - -
CHEMBL1222228 IC50 - -
CHEMBL1222227 IC50 - -
CHEMBL1222226 IC50 - -
CHEMBL1222131 IC50 - -
CHEMBL1222024 IC50 - -
CHEMBL1222023 IC50 - -