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Assay Detail

CHEMBL1228686

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Binding
Binding affinity to cathepsin B in crude rat liver homogenates using [125I]DCG-04 based competition protein labeling by SDS-PAGE method
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Liver
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cathepsin B (CHEMBL2602)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Activity-based probes that target diverse cysteine protease families.
Kato D, Boatright KM, Berger AB, Nazif T, Blum G, Ryan C, Chehade KA, Salvesen GS, Bogyo M.
Nat Chem Biol (2005) 1 : 33 -38
PubMed 16407991 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 6.92 8.14

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1221752 1 8.14
CHEMBL1221749 1 7.82
CHEMBL1221748 1 6.92
CHEMBL1221751 1 6.66
CHEMBL1221750 1 6.04
CHEMBL1221693 1 5.92

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1221752 IC50 = 7.3 nM 8.14
CHEMBL1221749 IC50 = 15.0 nM 7.82
CHEMBL1221748 IC50 = 120.0 nM 6.92
CHEMBL1221751 IC50 = 220.0 nM 6.66
CHEMBL1221750 IC50 = 920.0 nM 6.04
CHEMBL1221693 IC50 = 1200.0 nM 5.92