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Assay Detail

CHEMBL1244633

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to His-tagged Vibrio cholerae recombinant MTAN expressed in Escherichia coli B21
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Vibrio cholerae
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (CHEMBL1250374)
Type SINGLE PROTEIN
Organism Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

Publication

Transition state analogs of 5'-methylthioadenosine nucleosidase disrupt quorum sensing.
Gutierrez JA, Crowder T, Rinaldo-Matthis A, Ho MC, Almo SC, Schramm VL.
Nat Chem Biol (2009) 5 : 251 -257
PubMed 19270684 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 8 9.26 10.19

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1232180 1 10.19
CHEMBL1242854 1 10.15
CHEMBL405346 1 10.14
CHEMBL1230265 1 9.48
CHEMBL1242855 1 8.80
CHEMBL1241493 1 8.68
CHEMBL1241494 1 8.66
CHEMBL1195586 1 8.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1232180 Kd = 0.064 nM 10.19
CHEMBL1242854 Kd = 0.07 nM 10.15
CHEMBL405346 Kd = 0.073 nM 10.14
CHEMBL1230265 Kd = 0.33 nM 9.48
CHEMBL1242855 Kd = 1.6 nM 8.80
CHEMBL1241493 Kd = 2.1 nM 8.68
CHEMBL1241494 Kd = 2.2 nM 8.66
CHEMBL1195586 Kd = 10.0 nM 8.00