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Assay Detail

CHEMBL1246065

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human GST-CDK1/cyclin B1 expressed in baculovirus using [gamma-33P]ATP after 45 mins by liquid scintillation counting
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Cyclin-dependent kinase 1/cyclin B1 (CHEMBL1907602)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Discovery of novel CDK1 inhibitors by combining pharmacophore modeling, QSAR analysis and in silico screening followed by in vitro bioassay.
Al-Sha'er MA, Taha MO.
Eur J Med Chem (2010) 45 : 4316 -4330
PubMed 20638755 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 6.33 7.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL428690 ALVOCIDIB 3.0 1 7.52
CHEMBL296586 KENPAULLONE 1 6.40
CHEMBL1242973 BUTYROLACTONE I 1 6.22
CHEMBL280074 OLOMOUCINE 1 5.16

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL428690 ALVOCIDIB IC50 = 30.0 nM 7.52
CHEMBL296586 KENPAULLONE IC50 = 400.0 nM 6.40
CHEMBL1242973 BUTYROLACTONE I IC50 = 600.0 nM 6.22
CHEMBL280074 OLOMOUCINE IC50 = 7000.0 nM 5.16