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Assay Detail

CHEMBL1259734

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at mineralocorticoid receptor-LBD in human U2OS cells transfected with Gal4-DBD assessed as inhibition of transactivation activity after 18 hrs by luciferase reporter gene assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type U2OS
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mineralocorticoid receptor (CHEMBL1994)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and biochemical characterization of a series of 17α-perfluoroalkylated estradiols as selective ligands for estrogen receptor α.
Eignerová B, Sedlák D, Dracínský M, Bartunek P, Kotora M.
J Med Chem (2010) 53 : 6947 -6953
PubMed 20812681 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 6.25 8.03

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL273453 ALDOSTERONE 2.0 1 8.03
CHEMBL1258901 1 5.42
CHEMBL1257167 1 5.29
CHEMBL1257168 1 -
CHEMBL1257284 1 -
CHEMBL1256104 1 -
CHEMBL1256105 1 -
CHEMBL1256103 1 -
CHEMBL1258902 1 -
CHEMBL1256102 1 -
CHEMBL1258783 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL273453 ALDOSTERONE IC50 = 9.268 nM 8.03
CHEMBL1258901 IC50 = 3810.66 nM 5.42
CHEMBL1257167 IC50 = 5140.44 nM 5.29
CHEMBL1257168 IC50 < 14421.15 nM -
CHEMBL1257284 IC50 < 15848.93 nM -
CHEMBL1256104 IC50 < 15848.93 nM -
CHEMBL1256105 IC50 < 15848.93 nM -
CHEMBL1256103 IC50 < 4819.48 nM -
CHEMBL1258902 IC50 < 12560.3 nM -
CHEMBL1256102 IC50 < 5357.97 nM -
CHEMBL1258783 IC50 < 6652.73 nM -