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Assay Detail

CHEMBL1272437

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant 11beta-HSD1 expressed in HEK293 cells assessed as conversion of [1,2-3H]-cortisone to cortisol by scintillation counting
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

11-beta-hydroxysteroid dehydrogenase 1 (CHEMBL4235)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis of novel 3-amino and 29-hydroxamic acid derivatives of glycyrrhetinic acid as selective 11β-hydroxysteroid dehydrogenase 2 inhibitors.
Stanetty C, Czollner L, Koller I, Shah P, Gaware R, Cunha TD, Odermatt A, Jordis U, Kosma P, Classen-Houben D.
Bioorg Med Chem (2010) 18 : 7522 -7541
PubMed 20851614 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 6.19 7.37

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1269877 1 7.37
CHEMBL1271410 1 7.21
CHEMBL493512 1 6.21
CHEMBL1270688 1 6.20
CHEMBL230006 ENOXOLONE 2.0 1 6.11
CHEMBL1270093 1 6.08
CHEMBL1270994 1 6.03
CHEMBL1269273 1 6.00
CHEMBL1271303 1 5.94
CHEMBL1270589 1 5.82
CHEMBL207413 ACETOXOLONE -1.0 1 5.15

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1269877 IC50 = 42.7 nM 7.37
CHEMBL1271410 IC50 = 61.9 nM 7.21
CHEMBL493512 IC50 = 619.0 nM 6.21
CHEMBL1270688 IC50 = 626.0 nM 6.20
CHEMBL230006 ENOXOLONE IC50 = 778.0 nM 6.11
CHEMBL1270093 IC50 = 825.0 nM 6.08
CHEMBL1270994 IC50 = 929.0 nM 6.03
CHEMBL1269273 IC50 = 1012.0 nM 6.00
CHEMBL1271303 IC50 = 1157.0 nM 5.94
CHEMBL1270589 IC50 = 1508.0 nM 5.82
CHEMBL207413 ACETOXOLONE IC50 = 7138.0 nM 5.15