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Assay Detail

CHEMBL1273067

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of bovine eNOS assessed as nitric oxide mediated oxidation of oxyhemoglobin to methemoglobin
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Bos taurus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Nitric oxide synthase 3 (CHEMBL4802)
Type SINGLE PROTEIN
Organism Bos taurus

Publication

N-Substituted acetamidines and 2-methylimidazole derivatives as selective inhibitors of neuronal nitric oxide synthase.
Maccallini C, Patruno A, Lannutti F, Ammazzalorso A, De Filippis B, Fantacuzzi M, Franceschelli S, Giampietro L, Masella S, Felaco M, Re N, Amoroso R.
Bioorg Med Chem Lett (2010) 20 : 6495 -6499
PubMed 20933416 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 4.04 4.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1269155 EFETOZOLE 2.0 1 4.10
CHEMBL450583 1 4.10
CHEMBL1269154 1 4.05
CHEMBL1269156 1 4.00
CHEMBL1269105 1 4.00
CHEMBL1269103 1 4.00
CHEMBL107251 1400W 1 -
CHEMBL1269106 1 -
CHEMBL1269104 1 -
CHEMBL448703 1 -
CHEMBL462334 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1269155 EFETOZOLE IC50 = 80000.0 nM 4.10
CHEMBL450583 IC50 = 80000.0 nM 4.10
CHEMBL1269154 IC50 = 90000.0 nM 4.05
CHEMBL1269156 IC50 = 100000.0 nM 4.00
CHEMBL1269105 IC50 = 100000.0 nM 4.00
CHEMBL1269103 IC50 = 100000.0 nM 4.00
CHEMBL107251 1400W IC50 = 1100000.0 nM -
CHEMBL1269106 IC50 = 400000.0 nM -
CHEMBL1269104 IC50 = 350000.0 nM -
CHEMBL448703 IC50 = 300000.0 nM -
CHEMBL462334 IC50 = 350000.0 nM -