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Assay Detail

CHEMBL1274492

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of chymotrypsin-like activity of human 20s proteasome beta5 subunit in human erythrocyte assessed as hydrolysis of Ac-WLA-AMC fluorogenic substrate
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Optimization of a series of dipeptides with a P3 threonine residue as non-covalent inhibitors of the chymotrypsin-like activity of the human 20S proteasome.
Blackburn C, Barrett C, Blank JL, Bruzzese FJ, Bump N, Dick LR, Fleming P, Garcia K, Hales P, Hu Z, Jones M, Liu JX, Sappal DS, Sintchak MD, Tsu C, Gigstad KM.
Bioorg Med Chem Lett (2010) 20 : 6581 -6586
PubMed 20875739 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 24 7.02 8.14

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1270724 1 8.14
CHEMBL1269196 1 8.13
CHEMBL325041 BORTEZOMIB 3.0 1 8.09
CHEMBL1269195 1 7.89
CHEMBL184693 1 7.82
CHEMBL1269181 1 7.80
CHEMBL1269188 1 7.70
CHEMBL1269186 1 7.70
CHEMBL1269187 1 7.60
CHEMBL1271031 1 7.51
CHEMBL1269193 1 7.17
CHEMBL1269185 1 7.13
CHEMBL1270523 1 7.12
CHEMBL1270931 1 7.00
CHEMBL1270625 1 6.92
CHEMBL1269194 1 6.68
CHEMBL1270829 1 6.36
CHEMBL1269184 1 6.35
CHEMBL1269183 1 6.26
CHEMBL1269182 1 5.96
CHEMBL1269189 1 5.51
CHEMBL1269192 1 5.50
CHEMBL1269190 1 5.04
CHEMBL1269191 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1270724 IC50 = 7.3 nM 8.14
CHEMBL1269196 IC50 = 7.4 nM 8.13
CHEMBL325041 BORTEZOMIB IC50 = 8.2 nM 8.09
CHEMBL1269195 IC50 = 13.0 nM 7.89
CHEMBL184693 IC50 = 15.0 nM 7.82
CHEMBL1269181 IC50 = 16.0 nM 7.80
CHEMBL1269188 IC50 = 20.0 nM 7.70
CHEMBL1269186 IC50 = 20.0 nM 7.70
CHEMBL1269187 IC50 = 25.0 nM 7.60
CHEMBL1271031 IC50 = 31.0 nM 7.51
CHEMBL1269193 IC50 = 68.0 nM 7.17
CHEMBL1269185 IC50 = 74.0 nM 7.13
CHEMBL1270523 IC50 = 75.0 nM 7.12
CHEMBL1270931 IC50 = 100.0 nM 7.00
CHEMBL1270625 IC50 = 120.0 nM 6.92
CHEMBL1269194 IC50 = 210.0 nM 6.68
CHEMBL1270829 IC50 = 440.0 nM 6.36
CHEMBL1269184 IC50 = 450.0 nM 6.35
CHEMBL1269183 IC50 = 550.0 nM 6.26
CHEMBL1269182 IC50 = 1100.0 nM 5.96
CHEMBL1269189 IC50 = 3100.0 nM 5.51
CHEMBL1269192 IC50 = 3200.0 nM 5.50
CHEMBL1269190 IC50 = 9100.0 nM 5.04
CHEMBL1269191 IC50 > 100000.0 nM -