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Assay Detail

CHEMBL1275013

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of PHLPP expressed in 24 hrs serum starved Cos7 cells assessed as increase of Akt phosphorylation after 30 mins by immunoblotting
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type COS-7
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Discovery of small molecule inhibitors of the PH domain leucine-rich repeat protein phosphatase (PHLPP) by chemical and virtual screening.
Sierecki E, Sinko W, McCammon JA, Newton AC.
J Med Chem (2010) 53 : 6899 -6911
PubMed 20836557 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 4.34 4.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL471225 1 4.52
CHEMBL1270266 1 4.16

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL471225 IC50 = 30000.0 nM 4.52
CHEMBL1270266 IC50 = 70000.0 nM 4.16