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Assay Detail

CHEMBL1285503

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Competitive inhibition of mushroom tyrosinase after 15 mins by Lineweaver-Burke plot analysis
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Agaricus bisporus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosinase (CHEMBL3318)
Type SINGLE PROTEIN
Organism Agaricus bisporus

Publication

Structural insights into the hot spot amino acid residues of mushroom tyrosinase for the bindings of thujaplicins.
Takahashi S, Kamiya T, Saeki K, Nezu T, Takeuchi S, Takasawa R, Sunaga S, Yoshimori A, Ebizuka S, Abe T, Tanuma S.
Bioorg Med Chem (2010) 18 : 8112 -8118
PubMed 20947360 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 3 6.80 7.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1275999 GAMMA-THUJAPLICIN 1 7.70
CHEMBL48310 HINOKITIOL 1 7.22
CHEMBL1275969 ALPHA-THUJAPLICIN 1 5.48

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1275999 GAMMA-THUJAPLICIN Ki = 20.0 nM 7.70
CHEMBL48310 HINOKITIOL Ki = 60.0 nM 7.22
CHEMBL1275969 ALPHA-THUJAPLICIN Ki = 3300.0 nM 5.48