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Assay Detail

CHEMBL1286451

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of mouse NaV1.8 expressed in HEK293 cells by isotopic efflux assay
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium channel protein type 10 subunit alpha (CHEMBL5158)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Discovery and biological evaluation of potent, selective, orally bioavailable, pyrazine-based blockers of the Na(v)1.8 sodium channel with efficacy in a model of neuropathic pain.
Scanio MJ, Shi L, Drizin I, Gregg RJ, Atkinson RN, Thomas JB, Johnson MS, Chapman ML, Liu D, Krambis MJ, Liu Y, Shieh CC, Zhang X, Simler GH, Joshi S, Honore P, Marsh KC, Knox A, Werness S, Antonio B, Krafte DS, Jarvis MF, Faltynek CR, Marron BE, Kort ME.
Bioorg Med Chem (2010) 18 : 7816 -7825
PubMed 20965738 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 6.04 7.25

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1277877 1 7.25
CHEMBL1271106 1 7.24
CHEMBL1278223 1 7.18
CHEMBL1277959 1 6.96
CHEMBL1277876 1 6.68
CHEMBL1278224 1 6.62
CHEMBL1278044 1 6.33
CHEMBL1276882 1 6.32
CHEMBL1277514 1 6.31
CHEMBL1277958 1 6.19
CHEMBL1278045 1 6.14
CHEMBL250699 1 6.07
CHEMBL1277513 1 6.02
CHEMBL1276881 1 5.99
CHEMBL1277512 1 5.97
CHEMBL1277606 1 5.87
CHEMBL1277700 1 5.60
CHEMBL1277607 1 5.57
CHEMBL1277792 1 5.38
CHEMBL1278138 1 5.03
CHEMBL1277699 1 4.96
CHEMBL1277608 1 4.75
CHEMBL1277793 1 4.54

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1277877 IC50 = 56.0 nM 7.25
CHEMBL1271106 IC50 = 58.0 nM 7.24
CHEMBL1278223 IC50 = 66.0 nM 7.18
CHEMBL1277959 IC50 = 110.0 nM 6.96
CHEMBL1277876 IC50 = 210.0 nM 6.68
CHEMBL1278224 IC50 = 240.0 nM 6.62
CHEMBL1278044 IC50 = 470.0 nM 6.33
CHEMBL1276882 IC50 = 480.0 nM 6.32
CHEMBL1277514 IC50 = 490.0 nM 6.31
CHEMBL1277958 IC50 = 650.0 nM 6.19
CHEMBL1278045 IC50 = 730.0 nM 6.14
CHEMBL250699 IC50 = 850.0 nM 6.07
CHEMBL1277513 IC50 = 960.0 nM 6.02
CHEMBL1276881 IC50 = 1030.0 nM 5.99
CHEMBL1277512 IC50 = 1070.0 nM 5.97
CHEMBL1277606 IC50 = 1340.0 nM 5.87
CHEMBL1277700 IC50 = 2500.0 nM 5.60
CHEMBL1277607 IC50 = 2680.0 nM 5.57
CHEMBL1277792 IC50 = 4140.0 nM 5.38
CHEMBL1278138 IC50 = 9400.0 nM 5.03
CHEMBL1277699 IC50 = 11000.0 nM 4.96
CHEMBL1277608 IC50 = 18000.0 nM 4.75
CHEMBL1277793 IC50 = 29100.0 nM 4.54