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Assay Detail

CHEMBL1613862

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
PUBCHEM_BIOASSAY: Luminescence-based cell-based dose response assay to identify inhibitors of NADPH oxidase 1 (NOX1): Cherry picks 2. (Class of assay: confirmatory) [Related pubchem assays: 2808 (Primary screen (NOX1 inhibitors, purchased analogs)), 1796 (Summary AID (NOX1 inhibitors)), 434974 (Psychoactive Drug Screening Program primary screen (NOX1 inhibitors)), 434953 (Psychoactive Drug Screening Program secondary screen (NOX1 inhibitors)), 2773 (Primary screen (NOX1 inhibitors, synthesized analogs set 3)), 2752 (Primary screen (NOX1 inhibitors, synthesized analogs set 2)), 2532 (Dose response (NOX1 inhibitors, HEK/293 cells)), 2819 (Primary screen (NOX1 inhibitors, purchased analogs set 2)), 2664 (Primary screen (NOX1 inhibitors, synthesized analogs)), 1792 (Primary Screen (NOX1 inhibitors)), 2545 (Confirmation screen (NOX1 inhibitors, HEK/293 cells)), 2539 (Counterscreen (NOX2, NOX3, NOX4 inhibitors)), 2556 (Counterscreen (Xanthine oxidase inhibitors)), 2538 (Dose response screen (NOX1 inhibitors)), 2541 (Confirmation screen (NOX1 inhibitors)), 1823 (Counterscreen (luminal))]
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Category confirmatory
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

NADPH oxidase 1 (CHEMBL1287628)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

PubChem BioAssay data set

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 6.42 6.64

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1473456 1 6.64
CHEMBL541018 1 6.50
CHEMBL441558 1 6.43
CHEMBL1517979 1 6.36
CHEMBL1434771 1 6.17

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1473456 IC50 = 230.0 nM 6.64
CHEMBL541018 IC50 = 320.0 nM 6.50
CHEMBL441558 IC50 = 370.0 nM 6.43
CHEMBL1517979 IC50 = 440.0 nM 6.36
CHEMBL1434771 IC50 = 670.0 nM 6.17