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Assay Detail

CHEMBL1640688

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of HDAC in human HeLa cell nuclear extracts
18
Total Activities
15
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HeLa
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Histone deacetylase (CHEMBL2093865)
Type PROTEIN FAMILY
Organism Homo sapiens

Publication

Novel chimeric histone deacetylase inhibitors: a series of lapatinib hybrides as potent inhibitors of epidermal growth factor receptor (EGFR), human epidermal growth factor receptor 2 (HER2), and histone deacetylase activity.
Mahboobi S, Sellmer A, Winkler M, Eichhorn E, Pongratz H, Ciossek T, Baer T, Maier T, Beckers T.
J Med Chem (2010) 53 : 8546 -8555
PubMed 21080629 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 5.91 7.33
Emax 3 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1630109 1 7.33
CHEMBL98 VORINOSTAT 4.0 1 7.16
CHEMBL1630118 2 6.37
CHEMBL1630107 1 6.20
CHEMBL1630117 2 5.08
CHEMBL514671 1 4.96
CHEMBL1630108 1 4.30
CHEMBL1630110 1 -
CHEMBL1630111 1 -
CHEMBL1630112 2 -
CHEMBL1630113 1 -
CHEMBL1630114 1 -
CHEMBL1630115 1 -
CHEMBL1630116 1 -
CHEMBL554 LAPATINIB 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1630109 IC50 = 47.0 nM 7.33
CHEMBL98 VORINOSTAT IC50 = 70.0 nM 7.16
CHEMBL1630118 IC50 = 430.0 nM 6.37
CHEMBL1630107 IC50 = 630.0 nM 6.20
CHEMBL1630117 IC50 = 8300.0 nM 5.08
CHEMBL514671 IC50 = 11000.0 nM 4.96
CHEMBL1630108 IC50 = 50000.0 nM 4.30
CHEMBL1630111 IC50 > 100000.0 nM -
CHEMBL1630112 IC50 > 10000.0 nM -
CHEMBL1630113 IC50 > 32000.0 nM -
CHEMBL1630114 IC50 > 32000.0 nM -
CHEMBL1630115 IC50 > 32000.0 nM -
CHEMBL1630116 IC50 > 32000.0 nM -
CHEMBL554 LAPATINIB IC50 > 100000.0 nM -
CHEMBL1630112 Emax = 20.0 % -
CHEMBL1630117 Emax = 30.0 % -
CHEMBL1630110 IC50 > 32000.0 nM -
CHEMBL1630118 Emax = 65.0 % -