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Assay Detail

CHEMBL1640706

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Cytotoxicity against human SKBR3 cells overexpressing HER2 by resazurin dye reduction assay
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type SK-BR-3
Confidence 1 — Organism
Curated By Autocuration

Target

SK-BR-3 (CHEMBL613834)
Type CELL-LINE
Organism Homo sapiens

Publication

Novel chimeric histone deacetylase inhibitors: a series of lapatinib hybrides as potent inhibitors of epidermal growth factor receptor (EGFR), human epidermal growth factor receptor 2 (HER2), and histone deacetylase activity.
Mahboobi S, Sellmer A, Winkler M, Eichhorn E, Pongratz H, Ciossek T, Baer T, Maier T, Beckers T.
J Med Chem (2010) 53 : 8546 -8555
PubMed 21080629 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 6.11 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL554 LAPATINIB 4.0 1 8.70
CHEMBL1630113 1 6.58
CHEMBL1630117 1 6.57
CHEMBL1630110 1 6.48
CHEMBL1630109 1 6.46
CHEMBL1630118 1 6.38
CHEMBL1630107 1 6.29
CHEMBL1630112 1 6.21
CHEMBL1630108 1 5.96
CHEMBL1630111 1 5.89
CHEMBL98 VORINOSTAT 4.0 1 5.58
CHEMBL1630116 1 5.36
CHEMBL1630115 1 4.77
CHEMBL1630114 1 4.30

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL554 LAPATINIB IC50 = 2.0 nM 8.70
CHEMBL1630113 IC50 = 260.0 nM 6.58
CHEMBL1630117 IC50 = 270.0 nM 6.57
CHEMBL1630110 IC50 = 330.0 nM 6.48
CHEMBL1630109 IC50 = 350.0 nM 6.46
CHEMBL1630118 IC50 = 420.0 nM 6.38
CHEMBL1630107 IC50 = 510.0 nM 6.29
CHEMBL1630112 IC50 = 620.0 nM 6.21
CHEMBL1630108 IC50 = 1100.0 nM 5.96
CHEMBL1630111 IC50 = 1300.0 nM 5.89
CHEMBL98 VORINOSTAT IC50 = 2600.0 nM 5.58
CHEMBL1630116 IC50 = 4400.0 nM 5.36
CHEMBL1630115 IC50 = 17000.0 nM 4.77
CHEMBL1630114 IC50 = 50000.0 nM 4.30