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Assay Detail

CHEMBL1646311

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiinflammatory activity in human PBMC assessed as inhibition of LPS-induced TNFalpha release treated for 5 mins before LPS challenge measured after 6 hrs by ELISA
25
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type PBMC
Confidence 1 — Organism
Curated By Autocuration

Target

PBMC (CHEMBL613107)
Type CELL-LINE
Organism Homo sapiens

Publication

Imidazo[4,5-d]thiazolo[5,4-b]pyridine based inhibitors of IKK2: synthesis, SAR, PK/PD and activity in a preclinical model of rheumatoid arthritis.
Dyckman AJ, Langevine CM, Quesnelle C, Kempson J, Guo J, Gill P, Spergel SH, Watterson SH, Li T, Nirschl DS, Gillooly KM, Pattoli MA, McIntyre KW, Chen L, McKinnon M, Dodd JH, Barrish JC, Burke JR, Pitts WJ.
Bioorg Med Chem Lett (2011) 21 : 383 -386
PubMed 21087862 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 25 6.31 7.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1642545 1 7.10
CHEMBL1642549 1 6.77
CHEMBL1642546 1 6.66
CHEMBL1642563 1 6.66
CHEMBL1642547 1 6.54
CHEMBL1642565 1 6.54
CHEMBL521643 1 6.51
CHEMBL454973 1 6.46
CHEMBL1642557 1 6.40
CHEMBL1642558 1 6.36
CHEMBL1642561 1 6.36
CHEMBL1642560 1 6.32
CHEMBL1642548 1 6.28
CHEMBL1642564 1 6.25
CHEMBL1642550 1 6.25
CHEMBL1642559 1 6.22
CHEMBL1642556 1 6.16
CHEMBL523189 1 6.14
CHEMBL1642562 1 6.03
CHEMBL249697 BMS-345541 1 6.00
CHEMBL1642554 1 6.00
CHEMBL1642551 1 5.87
CHEMBL1642552 1 5.31
CHEMBL1642555 1 -
CHEMBL1642553 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1642545 IC50 = 80.0 nM 7.10
CHEMBL1642549 IC50 = 170.0 nM 6.77
CHEMBL1642546 IC50 = 220.0 nM 6.66
CHEMBL1642563 IC50 = 220.0 nM 6.66
CHEMBL1642547 IC50 = 290.0 nM 6.54
CHEMBL1642565 IC50 = 290.0 nM 6.54
CHEMBL521643 IC50 = 310.0 nM 6.51
CHEMBL454973 IC50 = 350.0 nM 6.46
CHEMBL1642557 IC50 = 400.0 nM 6.40
CHEMBL1642558 IC50 = 440.0 nM 6.36
CHEMBL1642561 IC50 = 440.0 nM 6.36
CHEMBL1642560 IC50 = 480.0 nM 6.32
CHEMBL1642548 IC50 = 530.0 nM 6.28
CHEMBL1642564 IC50 = 560.0 nM 6.25
CHEMBL1642550 IC50 = 560.0 nM 6.25
CHEMBL1642559 IC50 = 600.0 nM 6.22
CHEMBL1642556 IC50 = 700.0 nM 6.16
CHEMBL523189 IC50 = 730.0 nM 6.14
CHEMBL1642562 IC50 = 940.0 nM 6.03
CHEMBL249697 BMS-345541 IC50 = 1000.0 nM 6.00
CHEMBL1642554 IC50 = 990.0 nM 6.00
CHEMBL1642551 IC50 = 1360.0 nM 5.87
CHEMBL1642552 IC50 = 4890.0 nM 5.31
CHEMBL1642555 IC50 > 10000.0 nM -
CHEMBL1642553 IC50 - -