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Assay Detail

CHEMBL1646345

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of rat 11beta-HSD1 assessed as inhibition of conversion of [3H]cortisone to [3H]cortisol by scintillation proximity assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

11-beta-hydroxysteroid dehydrogenase 1 (CHEMBL2391)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Synthesis and optimization of novel 4,4-disubstituted cyclohexylbenzamide derivatives as potent 11β-HSD1 inhibitors.
Sun D, Wang Z, Caille S, DeGraffenreid M, Gonzalez-Lopez de Turiso F, Hungate R, Jaen JC, Jiang B, Julian LD, Kelly R, McMinn DL, Kaizerman J, Rew Y, Sudom A, Tu H, Ursu S, Walker N, Willcockson M, Yan X, Ye Q, Powers JP.
Bioorg Med Chem Lett (2011) 21 : 405 -410
PubMed 21093258 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 7.02 7.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1642603 1 7.52
CHEMBL1642595 1 7.07
CHEMBL1642600 1 6.84
CHEMBL1642602 1 6.66

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1642603 IC50 = 30.0 nM 7.52
CHEMBL1642595 IC50 = 86.0 nM 7.07
CHEMBL1642600 IC50 = 144.0 nM 6.84
CHEMBL1642602 IC50 = 220.0 nM 6.66