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Assay Detail

CHEMBL1647736

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]clonidine from imidazoline I1 receptor in Sprague-Dawley rat kidney membrane after 45 mins by liquid scintillation counting
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Kidney
Subcellular Membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Nischarin (CHEMBL5221)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

3-[(Imidazolidin-2-yl)imino]indazole ligands with selectivity for the α(2)-adrenoceptor compared to the imidazoline I(1) receptor.
Sączewski F, Kornicka A, Hudson AL, Laird S, Scheinin M, Laurila JM, Rybczyńska A, Boblewski K, Lehmann A, Gdaniec M.
Bioorg Med Chem (2011) 19 : 321 -329
PubMed 21129985 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 5.11 5.74

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1641681 1 5.74
CHEMBL1641687 1 5.70
CHEMBL1641679 1 5.49
CHEMBL1641689 1 5.40
CHEMBL1641680 1 5.12
CHEMBL1641688 1 4.78
CHEMBL1641685 1 4.67
CHEMBL1641683 1 4.59
CHEMBL1641682 1 4.49
CHEMBL1641684 1 -
CHEMBL1641686 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1641681 IC50 = 1840.0 nM 5.74
CHEMBL1641687 IC50 = 2010.0 nM 5.70
CHEMBL1641679 IC50 = 3219.0 nM 5.49
CHEMBL1641689 IC50 = 4010.0 nM 5.40
CHEMBL1641680 IC50 = 7560.0 nM 5.12
CHEMBL1641688 IC50 = 16600.0 nM 4.78
CHEMBL1641685 IC50 = 21200.0 nM 4.67
CHEMBL1641683 IC50 = 25600.0 nM 4.59
CHEMBL1641682 IC50 = 32500.0 nM 4.49
CHEMBL1641684 IC50 > 100000.0 nM -
CHEMBL1641686 IC50 > 100000.0 nM -