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Assay Detail

CHEMBL1648152

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Transactivation of Gal4-fused human PPARalpha DNA binding domain expressed in african green monkey CV1 cells by luciferase reporter gene assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CV-1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Peroxisome proliferator-activated receptor alpha (CHEMBL239)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis of a novel human PPARδ selective agonist and its stimulatory effect on oligodendrocyte differentiation.
Sakuma S, Endo T, Kanda T, Nakamura H, Yamasaki S, Yamakawa T.
Bioorg Med Chem Lett (2011) 21 : 240 -244
PubMed 21112784 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 4 7.00 8.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL219586 GW590735 2.0 1 8.00
CHEMBL38943 GW501516 2.0 1 6.00
CHEMBL1644614 1 -
CHEMBL121 ROSIGLITAZONE 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL219586 GW590735 EC50 = 10.0 nM 8.00
CHEMBL38943 GW501516 EC50 = 990.0 nM 6.00
CHEMBL1644614 EC50 > 10000.0 nM -
CHEMBL121 ROSIGLITAZONE EC50 > 10000.0 nM -