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Assay Detail

CHEMBL1648428

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Functional
Agonist activity at human PPARdelta expressed in HepG2 cells co-transfected with PPRE3-TK-luc assessed as beta-galactosidase activity after 20 to 22 hrs by luciferase based transactivation assay
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HepG2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Peroxisome proliferator-activated receptor delta (CHEMBL3979)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Effect of structurally constrained oxime-ether linker on PPAR subtype selectivity: Discovery of a novel and potent series of PPAR-pan agonists.
Makadia P, Shah SR, Pingali H, Zaware P, Patel D, Pola S, Thube B, Priyadarshini P, Suthar D, Shah M, Giri S, Trivedi C, Jain M, Patel P, Bahekar R.
Bioorg Med Chem (2011) 19 : 771 -782
PubMed 21215640 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 7 6.65 8.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL38943 GW501516 2.0 1 8.92
CHEMBL1644299 1 8.00
CHEMBL1644310 1 6.52
CHEMBL1644304 1 6.16
CHEMBL1644301 1 6.10
CHEMBL1644305 1 6.10
CHEMBL264374 BEZAFIBRATE 3.0 1 4.72

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL38943 GW501516 EC50 = 1.2 nM 8.92
CHEMBL1644299 EC50 = 10.0 nM 8.00
CHEMBL1644310 EC50 = 300.0 nM 6.52
CHEMBL1644304 EC50 = 700.0 nM 6.16
CHEMBL1644301 EC50 = 800.0 nM 6.10
CHEMBL1644305 EC50 = 800.0 nM 6.10
CHEMBL264374 BEZAFIBRATE EC50 = 18900.0 nM 4.72