Assay Detail
Binding
CHEMBL1655217
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Transactivation of Gal4-fused human PPARalpha expressed in HEK293 cells after 16 to 20 hrs by luciferase reporter gene assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | HEK293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Peroxisome proliferator-activated receptor alpha (CHEMBL239) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Design, synthesis, and structural analysis of phenylpropanoic acid-type PPARγ-selective agonists: discovery of reversed stereochemistry-activity relationship.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 6 | 6.85 | 7.92 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL202118 | — | — | 1 | 7.92 |
| CHEMBL402840 | — | — | 1 | 7.27 |
| CHEMBL382655 | — | — | 1 | 6.82 |
| CHEMBL375471 | — | — | 1 | 6.60 |
| CHEMBL272137 | — | — | 1 | 6.30 |
| CHEMBL427071 | — | — | 1 | 6.21 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL202118 | — | EC50 | = | 12.0 | nM | 7.92 |
| CHEMBL402840 | — | EC50 | = | 54.0 | nM | 7.27 |
| CHEMBL382655 | — | EC50 | = | 150.0 | nM | 6.82 |
| CHEMBL375471 | — | EC50 | = | 250.0 | nM | 6.60 |
| CHEMBL272137 | — | EC50 | = | 500.0 | nM | 6.30 |
| CHEMBL427071 | — | EC50 | = | 620.0 | nM | 6.21 |