Assay Detail
Binding
CHEMBL1664617
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human recombinant PDE5A-mediated hydrolysis of cGMP after 30 mins by fluorescence polarization assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
cGMP-specific 3',5'-cyclic phosphodiesterase (CHEMBL1827) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Synthesis and molecular modeling of novel tetrahydro-β-carboline derivatives with phosphodiesterase 5 inhibitory and anticancer properties.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 4 | 8.34 | 8.70 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1650866 | — | — | 1 | 8.70 |
| CHEMBL779 | TADALAFIL | 4.0 | 1 | 8.52 |
| CHEMBL1650676 | — | — | 1 | 8.15 |
| CHEMBL1650673 | — | — | 1 | 8.00 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1650866 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL779 | TADALAFIL | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL1650676 | — | IC50 | = | 7.0 | nM | 8.15 |
| CHEMBL1650673 | — | IC50 | = | 10.0 | nM | 8.00 |