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Assay Detail

CHEMBL1667096

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Escherichia coli MC4100 Beta-Lactamase CMY-2 using cephalothin as reporter substrate
8
Total Activities
8
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Escherichia coli
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Extended-spectrum properties of CMY-30, a Val211Gly mutant of CMY-2 cephalosporinase.
Kotsakis SD, Papagiannitsis CC, Tzelepi E, Tzouvelekis LS, Miriagou V.
Antimicrob Agents Chemother (2009) 53 : 3520 -3523
PubMed 19470510 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 7 7.76 9.70
Km 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL891 CLOXACILLIN 4.0 1 9.70
CHEMBL158 AZTREONAM 4.0 1 8.68
CHEMBL1730 CEFOTAXIME 4.0 1 8.30
CHEMBL44354 CEFTAZIDIME 4.0 1 7.70
CHEMBL333078 1 7.00
CHEMBL996 CEFOXITIN 4.0 1 6.82
CHEMBL404 TAZOBACTAM 4.0 1 6.10
CHEMBL29 PENICILLIN G 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL891 CLOXACILLIN Ki = 0.2 nM 9.70
CHEMBL158 AZTREONAM Ki = 2.1 nM 8.68
CHEMBL1730 CEFOTAXIME Ki = 5.0 nM 8.30
CHEMBL44354 CEFTAZIDIME Ki = 20.0 nM 7.70
CHEMBL333078 Ki = 100.0 nM 7.00
CHEMBL996 CEFOXITIN Ki = 150.0 nM 6.82
CHEMBL404 TAZOBACTAM Ki = 800.0 nM 6.10
CHEMBL29 PENICILLIN G Km = 600.0 nM -