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Assay Detail

CHEMBL1667348

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Functional
Antimalarial activity against pyrimethamine-sensitive Plasmodium falciparum 3D7 expressing wild type DHFR by [3H]hypoxanthine incorporation assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Plasmodium falciparum
Confidence 1 — Organism
Curated By Autocuration

Target

Plasmodium falciparum (CHEMBL364)
Type ORGANISM
Organism Plasmodium falciparum

Publication

In vitro activity of antifolate and polymorphism in dihydrofolate reductase of Plasmodium falciparum isolates from the Kenyan coast: emergence of parasites with Ile-164-Leu mutation.
Kiara SM, Okombo J, Masseno V, Mwai L, Ochola I, Borrmann S, Nzila A.
Antimicrob Agents Chemother (2009) 53 : 3793 -3798
PubMed 19528269 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 8.77 10.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL92583 CHLORCYCLOGUANIL 1 10.96
CHEMBL129788 1 10.82
CHEMBL119 TRIMETREXATE 4.0 1 8.72
CHEMBL36 PYRIMETHAMINE 4.0 1 8.41
CHEMBL34259 METHOTREXATE 4.0 1 7.30
CHEMBL22 TRIMETHOPRIM 4.0 1 6.39

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL92583 CHLORCYCLOGUANIL IC50 = 0.011 nM 10.96
CHEMBL129788 IC50 = 0.015 nM 10.82
CHEMBL119 TRIMETREXATE IC50 = 1.9 nM 8.72
CHEMBL36 PYRIMETHAMINE IC50 = 3.9 nM 8.41
CHEMBL34259 METHOTREXATE IC50 = 49.9 nM 7.30
CHEMBL22 TRIMETHOPRIM IC50 = 406.7 nM 6.39