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Assay Detail

CHEMBL1763100

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of RNA synthesis in mouse L1210 cells assessed as [5-3H]uridine incorporation in to RNA preincubated for 24 hrs by liquid scintillation counting
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type L1210
Confidence 1 — Organism
Curated By Autocuration

Target

L1210 (CHEMBL386)
Type CELL-LINE
Organism Mus musculus

Publication

Synthesis and biological evaluation of unsaturated keto and exomethylene D-arabinopyranonucleoside analogs: novel 5-fluorouracil analogs that target thymidylate synthase.
Tzioumaki N, Manta S, Tsoukala E, Vande Voorde J, Liekens S, Komiotis D, Balzarini J.
Eur J Med Chem (2011) 46 : 993 -1005
PubMed 21330014 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 4.73 5.31

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL185 FLUOROURACIL 4.0 1 5.31
CHEMBL1762220 1 4.80
CHEMBL1762225 1 4.08
CHEMBL1762230 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL185 FLUOROURACIL IC50 = 4900.0 nM 5.31
CHEMBL1762220 IC50 = 16000.0 nM 4.80
CHEMBL1762225 IC50 = 84000.0 nM 4.08
CHEMBL1762230 IC50 = 282000.0 nM -