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Assay Detail

CHEMBL1768784

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of PDK1-mediated RSK phosphorylation at Ser221 residue in human PC3 cells by ELISA
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type PC-3
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

3-phosphoinositide-dependent protein kinase 1 (CHEMBL2534)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-based design of potent and selective 3-phosphoinositide-dependent kinase-1 (PDK1) inhibitors.
Medina JR, Becker CJ, Blackledge CW, Duquenne C, Feng Y, Grant SW, Heerding D, Li WH, Miller WH, Romeril SP, Scherzer D, Shu A, Bobko MA, Chadderton AR, Dumble M, Gardiner CM, Gilbert S, Liu Q, Rabindran SK, Sudakin V, Xiang H, Brady PG, Campobasso N, Ward P, Axten JM.
J Med Chem (2011) 54 : 1871 -1895
PubMed 21341675 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 6.82 7.72

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1765750 1 7.72
CHEMBL1765743 1 7.52
CHEMBL1765742 1 7.06
CHEMBL1765744 1 6.99
CHEMBL1765751 1 6.94
CHEMBL1614777 1 6.82
CHEMBL1765740 1 6.53
CHEMBL1765739 1 6.50
CHEMBL1614775 1 6.48
CHEMBL1765741 1 6.23
CHEMBL1765738 1 6.18
CHEMBL1765736 1 -
CHEMBL1765737 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1765750 IC50 = 19.0 nM 7.72
CHEMBL1765743 IC50 = 30.0 nM 7.52
CHEMBL1765742 IC50 = 88.0 nM 7.06
CHEMBL1765744 IC50 = 102.0 nM 6.99
CHEMBL1765751 IC50 = 116.0 nM 6.94
CHEMBL1614777 IC50 = 151.0 nM 6.82
CHEMBL1765740 IC50 = 293.0 nM 6.53
CHEMBL1765739 IC50 = 316.0 nM 6.50
CHEMBL1614775 IC50 = 333.0 nM 6.48
CHEMBL1765741 IC50 = 594.0 nM 6.23
CHEMBL1765738 IC50 = 655.0 nM 6.18
CHEMBL1765736 IC50 > 29300.0 nM -
CHEMBL1765737 IC50 - -