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Assay Detail

CHEMBL1769659

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human recombinant histamine H1 receptor expressed in intact CHO cells assessed as inhibition of histamine-induced cellular calcium mobilization at 100 nM after 30 mins by FLIPR assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histamine H1 receptor (CHEMBL231)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

The discovery of phthalazinone-based human H1 and H3 single-ligand antagonists suitable for intranasal administration for the treatment of allergic rhinitis.
Procopiou PA, Browning C, Buckley JM, Clark KL, Fechner L, Gore PM, Hancock AP, Hodgson ST, Holmes DS, Kranz M, Looker BE, Morriss KM, Parton DL, Russell LJ, Slack RJ, Sollis SL, Vile S, Watts CJ.
J Med Chem (2011) 54 : 2183 -2195
PubMed 21381763 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 5 9.00 9.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL639 AZELASTINE 4.0 1 9.70
CHEMBL1767164 GSK-1004723 2.0 1 9.10
CHEMBL1767165 1 8.90
CHEMBL1767167 1 8.90
CHEMBL1767166 1 8.40

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL639 AZELASTINE Kd = 0.1995 nM 9.70
CHEMBL1767164 GSK-1004723 Kd = 0.7943 nM 9.10
CHEMBL1767165 Kd = 1.259 nM 8.90
CHEMBL1767167 Kd = 1.259 nM 8.90
CHEMBL1767166 Kd = 3.981 nM 8.40