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Assay Detail

CHEMBL1772295

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of gamma-secretase in human H4 cells expressing APP Swedish mutant assessed as inhibition of amyloid beta (1 to 40) production by whole cell assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type H4
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Gamma-secretase (CHEMBL2094135)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Design, synthesis, and in vivo characterization of a novel series of tetralin amino imidazoles as γ-secretase inhibitors: discovery of PF-3084014.
Brodney MA, Auperin DD, Becker SL, Bronk BS, Brown TM, Coffman KJ, Finley JE, Hicks CD, Karmilowicz MJ, Lanz TA, Liston D, Liu X, Martin BA, Nelson RB, Nolan CE, Oborski CE, Parker CP, Richter KE, Pozdnyakov N, Sahagan BG, Schachter JB, Sokolowski SA, Tate B, Wood DE, Wood KM, Van Deusen JW, Zhang L.
Bioorg Med Chem Lett (2011) 21 : 2637 -2640
PubMed 21269827 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 7.49 8.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1770916 NIROGACESTAT 4.0 1 8.92
CHEMBL1770911 1 8.54
CHEMBL1770912 1 8.48
CHEMBL1770914 1 8.34
CHEMBL1770917 1 8.31
CHEMBL1770910 1 8.27
CHEMBL1770915 1 8.16
CHEMBL1770907 1 7.91
CHEMBL1770913 1 7.71
CHEMBL1770909 1 7.71
CHEMBL1770908 1 7.33
CHEMBL1770905 1 7.27
CHEMBL1770906 1 6.81
CHEMBL1770904 1 6.57
CHEMBL1770901 1 6.51
CHEMBL1770903 1 6.32
CHEMBL1770902 1 6.12
CHEMBL1770900 1 5.51

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1770916 NIROGACESTAT IC50 = 1.2 nM 8.92
CHEMBL1770911 IC50 = 2.9 nM 8.54
CHEMBL1770912 IC50 = 3.3 nM 8.48
CHEMBL1770914 IC50 = 4.6 nM 8.34
CHEMBL1770917 IC50 = 4.9 nM 8.31
CHEMBL1770910 IC50 = 5.4 nM 8.27
CHEMBL1770915 IC50 = 6.9 nM 8.16
CHEMBL1770907 IC50 = 12.4 nM 7.91
CHEMBL1770913 IC50 = 19.3 nM 7.71
CHEMBL1770909 IC50 = 19.5 nM 7.71
CHEMBL1770908 IC50 = 46.8 nM 7.33
CHEMBL1770905 IC50 = 53.9 nM 7.27
CHEMBL1770906 IC50 = 155.0 nM 6.81
CHEMBL1770904 IC50 = 272.0 nM 6.57
CHEMBL1770901 IC50 = 310.0 nM 6.51
CHEMBL1770903 IC50 = 480.0 nM 6.32
CHEMBL1770902 IC50 = 754.0 nM 6.12
CHEMBL1770900 IC50 = 3100.0 nM 5.51