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Assay Detail

CHEMBL1780665

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Functional
Antagonist activity at P2X7 receptor expressed in HEK293 cells assessed as inhibition of ATP-induced YOPRO-1 uptake
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Cell Type HEK293
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

P2X purinoceptor 7 (CHEMBL4805)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Optimization of the physicochemical and pharmacokinetic attributes in a 6-azauracil series of P2X7 receptor antagonists leading to the discovery of the clinical candidate CE-224,535.
Duplantier AJ, Dombroski MA, Subramanyam C, Beaulieu AM, Chang SP, Gabel CA, Jordan C, Kalgutkar AS, Kraus KG, Labasi JM, Mussari C, Perregaux DG, Shepard R, Taylor TJ, Trevena KA, Whitney-Pickett C, Yoon K.
Bioorg Med Chem Lett (2011) 21 : 3708 -3711
PubMed 21565499 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 7.18 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1823817 CE-224535 2.0 1 8.40
CHEMBL560423 1 7.82
CHEMBL559968 1 6.52
CHEMBL559078 1 5.96

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1823817 CE-224535 IC50 = 4.0 nM 8.40
CHEMBL560423 IC50 = 15.0 nM 7.82
CHEMBL559968 IC50 = 300.0 nM 6.52
CHEMBL559078 IC50 = 1100.0 nM 5.96