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Assay Detail

CHEMBL1785171

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of NFkappa p65 in nuclear extract of human HeLa cells assessed as blockade of NFkappa p65 binding to biotinylated-consesus sequence by ELISA
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HeLa
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Transcription factor p65 (CHEMBL5533)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Cytotoxic and NF-κB inhibitory constituents of the stems of Cratoxylum cochinchinense and their semisynthetic analogues.
Ren Y, Matthew S, Lantvit DD, Ninh TN, Chai H, Fuchs JR, Soejarto DD, de Blanco EJ, Swanson SM, Kinghorn AD.
J Nat Prod (2011) 74 : 1117 -1125
PubMed 21428375 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 6.33 7.12

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL438139 ROCAGLAMIDE 1 7.12
CHEMBL1782240 1 5.54
CHEMBL1782239 COCHINENSOXANTHONE 1 -
CHEMBL323197 MANGOSTIN 1 -
CHEMBL260553 1 -
CHEMBL1782241 COCHINCHINONE A 1 -
CHEMBL1782242 1 -
CHEMBL465553 1 -
CHEMBL1782243 1 -
CHEMBL1782244 1 -
CHEMBL1782238 COCHINCHINOXANTHONE 1 -
CHEMBL1782245 1 -
CHEMBL1782059 1 -
CHEMBL1782246 1 -
CHEMBL1782247 1 -
CHEMBL1782248 1 -
CHEMBL1782582 1 -
CHEMBL1782583 1 -
CHEMBL1782584 1 -
CHEMBL464524 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL438139 ROCAGLAMIDE IC50 = 75.0 nM 7.12
CHEMBL1782240 IC50 = 2900.0 nM 5.54
CHEMBL1782239 COCHINENSOXANTHONE IC50 - -
CHEMBL323197 MANGOSTIN IC50 > 10000.0 nM -
CHEMBL260553 IC50 - -
CHEMBL1782241 COCHINCHINONE A IC50 > 10000.0 nM -
CHEMBL1782242 IC50 > 10000.0 nM -
CHEMBL465553 IC50 > 10000.0 nM -
CHEMBL1782243 IC50 > 10000.0 nM -
CHEMBL1782244 IC50 > 10000.0 nM -
CHEMBL1782238 COCHINCHINOXANTHONE IC50 > 10000.0 nM -
CHEMBL1782245 IC50 > 10000.0 nM -
CHEMBL1782059 IC50 - -
CHEMBL1782246 IC50 - -
CHEMBL1782247 IC50 > 10000.0 nM -
CHEMBL1782248 IC50 > 10000.0 nM -
CHEMBL1782582 IC50 > 10000.0 nM -
CHEMBL1782583 IC50 > 10000.0 nM -
CHEMBL1782584 IC50 > 10000.0 nM -
CHEMBL464524 IC50 > 10000.0 nM -