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Assay Detail

CHEMBL1787515

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of hypoxia-induced HIF1alpha transcriptional activity in HEK293 cells incubated for 16 hrs by hypoxia response element-driven luciferase reporter gene assay
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Hypoxia-inducible factor 1-alpha (CHEMBL4261)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

1-[4-(N-Benzylamino)phenyl]-3-phenylurea derivatives as a new class of hypoxia-inducible factor-1alpha inhibitors.
Uno M, Ban HS, Nakamura H.
Bioorg Med Chem Lett (2009) 19 : 3166 -3169
PubMed 19435661 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 4.61 5.14

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1782072 1 5.14
CHEMBL1783635 1 5.06
CHEMBL333985 1 5.04
CHEMBL1783638 1 4.82
CHEMBL1783625 1 4.76
CHEMBL1783634 1 4.73
CHEMBL1783637 1 4.66
CHEMBL1783627 1 4.64
CHEMBL1783636 1 4.53
CHEMBL1783631 1 4.49
CHEMBL1783622 1 4.49
CHEMBL1783624 1 4.45
CHEMBL1783633 1 4.41
CHEMBL1783629 1 4.38
CHEMBL1783628 1 4.29
CHEMBL1783626 1 4.28
CHEMBL1783623 1 4.16
CHEMBL1783632 1 -
CHEMBL1783630 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1782072 IC50 = 7210.0 nM 5.14
CHEMBL1783635 IC50 = 8620.0 nM 5.06
CHEMBL333985 IC50 = 9210.0 nM 5.04
CHEMBL1783638 IC50 = 15100.0 nM 4.82
CHEMBL1783625 IC50 = 17400.0 nM 4.76
CHEMBL1783634 IC50 = 18700.0 nM 4.73
CHEMBL1783637 IC50 = 22100.0 nM 4.66
CHEMBL1783627 IC50 = 23000.0 nM 4.64
CHEMBL1783636 IC50 = 29600.0 nM 4.53
CHEMBL1783631 IC50 = 32500.0 nM 4.49
CHEMBL1783622 IC50 = 32700.0 nM 4.49
CHEMBL1783624 IC50 = 35500.0 nM 4.45
CHEMBL1783633 IC50 = 39100.0 nM 4.41
CHEMBL1783629 IC50 = 41600.0 nM 4.38
CHEMBL1783628 IC50 = 51700.0 nM 4.29
CHEMBL1783626 IC50 = 52100.0 nM 4.28
CHEMBL1783623 IC50 = 69900.0 nM 4.16
CHEMBL1783632 IC50 > 100000.0 nM -
CHEMBL1783630 IC50 > 100000.0 nM -