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Assay Detail

CHEMBL1787847

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of catalytic activity of recombinant human AP-N transfected in human HEK-293 cells assessed as cleavage of substrate L-leucine-p-nitroanilide to L-leucine and p-nitroaniline measured every 5 mins for 10 to 50 mins by spectrophotometric analysis
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Aminopeptidase N (CHEMBL1907)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Potent macrocyclic inhibitors of insulin-regulated aminopeptidase (IRAP) by olefin ring-closing metathesis.
Andersson H, Demaegdt H, Johnsson A, Vauquelin G, Lindeberg G, Hallberg M, Erdelyi M, Karlen A, Hallberg A.
J Med Chem (2011) 54 : 3779 -3792
PubMed 21476495 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 15 5.58 6.62

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1782887 1 6.62
CHEMBL261120 ANGIOTENSIN IV 1 6.08
CHEMBL1782885 1 5.97
CHEMBL1782888 1 5.89
CHEMBL1782889 1 5.80
CHEMBL1782883 1 5.48
CHEMBL1782881 1 5.30
CHEMBL1782886 1 5.27
CHEMBL1782884 1 5.08
CHEMBL1782882 1 4.92
CHEMBL1782890 1 4.92
CHEMBL1782891 1 -
CHEMBL1782869 1 -
CHEMBL1782892 1 -
CHEMBL1782893 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1782887 Ki = 240.0 nM 6.62
CHEMBL261120 ANGIOTENSIN IV Ki = 841.0 nM 6.08
CHEMBL1782885 Ki = 1074.0 nM 5.97
CHEMBL1782888 Ki = 1299.0 nM 5.89
CHEMBL1782889 Ki = 1571.0 nM 5.80
CHEMBL1782883 Ki = 3283.0 nM 5.48
CHEMBL1782881 Ki = 4955.0 nM 5.30
CHEMBL1782886 Ki = 5413.0 nM 5.27
CHEMBL1782884 Ki = 8345.0 nM 5.08
CHEMBL1782882 Ki = 12000.0 nM 4.92
CHEMBL1782890 Ki = 12000.0 nM 4.92
CHEMBL1782891 Ki > 100000.0 nM -
CHEMBL1782869 Ki > 100000.0 nM -
CHEMBL1782892 Ki > 100000.0 nM -
CHEMBL1782893 Ki > 100000.0 nM -