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Assay Detail

CHEMBL1799009

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant cytosolic carbonic anhydrase 13 preincubated for 15 mins by stopped-flow CO2 hydration assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Carbonic anhydrase 13 (CHEMBL3912)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Conformational variability of different sulfonamide inhibitors with thienyl-acetamido moieties attributes to differential binding in the active site of cytosolic human carbonic anhydrase isoforms.
Biswas S, Aggarwal M, Güzel Ö, Scozzafava A, McKenna R, Supuran CT.
Bioorg Med Chem (2011) 19 : 3732 -3738
PubMed 21620713 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 5 7.28 7.77

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL20 ACETAZOLAMIDE 4.0 1 7.77
CHEMBL19 METHAZOLAMIDE 4.0 1 7.72
CHEMBL17 DICHLORPHENAMIDE 4.0 1 7.64
CHEMBL573127 1 6.90
CHEMBL574246 1 6.36

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL20 ACETAZOLAMIDE Ki = 17.0 nM 7.77
CHEMBL19 METHAZOLAMIDE Ki = 19.0 nM 7.72
CHEMBL17 DICHLORPHENAMIDE Ki = 23.0 nM 7.64
CHEMBL573127 Ki = 125.0 nM 6.90
CHEMBL574246 Ki = 437.0 nM 6.36