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Assay Detail

CHEMBL1799131

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant His-tagged full length LSD1 expressed in Escherichia coli BL21 (DE3) assessed as inactivation constant preincubated for 20 mins with substrate measured after 30 mins by AmplexRed based fluorescence quenching assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Lysine-specific histone demethylase 1A (CHEMBL6136)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Enantioselective synthesis of tranylcypromine analogues as lysine demethylase (LSD1) inhibitors.
Benelkebir H, Hodgkinson C, Duriez PJ, Hayden AL, Bulleid RA, Crabb SJ, Packham G, Ganesan A.
Bioorg Med Chem (2011) 19 : 3709 -3716
PubMed 21382717 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 11 4.75 5.43

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1795974 1 5.43
CHEMBL1795982 1 5.17
CHEMBL1795975 1 5.05
CHEMBL1795976 1 4.93
CHEMBL3989843 TRANYLCYPROMINE 4.0 1 4.60
CHEMBL257990 (+)-TRANYLCYPROMINE 1 4.58
CHEMBL1179 1 4.55
CHEMBL1795972 1 4.38
CHEMBL1795973 1 4.10
CHEMBL1795983 1 -
CHEMBL1795984 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1795974 Ki = 3700.0 nM 5.43
CHEMBL1795982 Ki = 6800.0 nM 5.17
CHEMBL1795975 Ki = 8900.0 nM 5.05
CHEMBL1795976 Ki = 11700.0 nM 4.93
CHEMBL3989843 TRANYLCYPROMINE Ki = 25000.0 nM 4.60
CHEMBL257990 (+)-TRANYLCYPROMINE Ki = 26600.0 nM 4.58
CHEMBL1179 Ki = 28100.0 nM 4.55
CHEMBL1795972 Ki = 41800.0 nM 4.38
CHEMBL1795973 Ki = 79600.0 nM 4.10
CHEMBL1795983 Ki > 100000.0 nM -
CHEMBL1795984 Ki > 100000.0 nM -