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Assay Detail

CHEMBL1799832

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant SIRT1 expressed in Escherichia coli incubated at 37 degC for 2 hrs by Fluor de Lys fluorescence assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

NAD-dependent protein deacetylase sirtuin-1 (CHEMBL4506)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Simplification of the tetracyclic SIRT1-selective inhibitor MC2141: coumarin- and pyrimidine-based SIRT1/2 inhibitors with different selectivity profile.
Rotili D, Carafa V, Tarantino D, Botta G, Nebbioso A, Altucci L, Mai A.
Bioorg Med Chem (2011) 19 : 3659 -3668
PubMed 21306905 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 4.61 5.08

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL596657 1 5.08
CHEMBL1797739 1 4.91
CHEMBL1797748 1 4.76
CHEMBL491960 CAMBINOL 1 4.24
CHEMBL490905 1 4.05
CHEMBL1797725 1 -
CHEMBL1797726 1 -
CHEMBL1797737 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL596657 IC50 = 8400.0 nM 5.08
CHEMBL1797739 IC50 = 12400.0 nM 4.91
CHEMBL1797748 IC50 = 17500.0 nM 4.76
CHEMBL491960 CAMBINOL IC50 = 57900.0 nM 4.24
CHEMBL490905 IC50 = 89800.0 nM 4.05
CHEMBL1797725 IC50 - -
CHEMBL1797726 IC50 - -
CHEMBL1797737 IC50 = 112000.0 nM -