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Assay Detail

CHEMBL1804380

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [125I]leuprorelin from recombinant human GnRH receptor expressed in CHO cells after 60 mins by X-ray counter in presence of 40% fetal bovine serum
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Gonadotropin-releasing hormone receptor (CHEMBL1855)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of 1-{4-[1-(2,6-difluorobenzyl)-5-[(dimethylamino)methyl]-3-(6-methoxypyridazin-3-yl)-2,4-dioxo-1,2,3,4-tetrahydrothieno[2,3-d]pyrimidin-6-yl]phenyl}-3-methoxyurea (TAK-385) as a potent, orally active, non-peptide antagonist of the human gonadotropin-releasing hormone receptor.
Miwa K, Hitaka T, Imada T, Sasaki S, Yoshimatsu M, Kusaka M, Tanaka A, Nakata D, Furuya S, Endo S, Hamamura K, Kitazaki T.
J Med Chem (2011) 54 : 4998 -5012
PubMed 21657270 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 9.35 9.62

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1800157 1 9.62
CHEMBL1800155 1 9.54
CHEMBL1800153 1 9.52
CHEMBL1800152 1 9.52
CHEMBL1800159 RELUGOLIX 4.0 1 9.48
CHEMBL1800668 1 9.30
CHEMBL1800158 1 9.26
CHEMBL1800156 1 9.10
CHEMBL22055 SUFUGOLIX 2.0 1 8.80

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1800157 IC50 = 0.24 nM 9.62
CHEMBL1800155 IC50 = 0.29 nM 9.54
CHEMBL1800153 IC50 = 0.3 nM 9.52
CHEMBL1800152 IC50 = 0.3 nM 9.52
CHEMBL1800159 RELUGOLIX IC50 = 0.33 nM 9.48
CHEMBL1800668 IC50 = 0.5 nM 9.30
CHEMBL1800158 IC50 = 0.55 nM 9.26
CHEMBL1800156 IC50 = 0.8 nM 9.10
CHEMBL22055 SUFUGOLIX IC50 = 1.6 nM 8.80