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Assay Detail

CHEMBL1805383

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of SHH pathway in mouse Shh Light2 cells after 40 hrs by Gli-dependent luciferase reporter gene assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type Shh Light II
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sonic hedgehog protein (CHEMBL5387)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Synthesis and biological evaluation of desmethylveramiline, a micromolar Hedgehog inhibitor.
Guerlet G, Spangenberg T, Mann A, Faure H, Ruat M.
Bioorg Med Chem Lett (2011) 21 : 3608 -3612
PubMed 21592788 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 6.49 7.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL480889 1 7.00
CHEMBL254129 CYCLOPAMINE 1 6.52
CHEMBL1803109 DESMETHYLVERAMILINE 1 5.96

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL480889 IC50 = 100.0 nM 7.00
CHEMBL254129 CYCLOPAMINE IC50 = 300.0 nM 6.52
CHEMBL1803109 DESMETHYLVERAMILINE IC50 = 1100.0 nM 5.96