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Assay Detail

CHEMBL1805588

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Competitive inhibition of histidine-tagged human recombinant IDO expressed in bacterial strain BL21 AI using L-Trptophan as substrate measured at 490 nm wavelength after 6 min by colorimetric assay
2
Total Activities
2
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Indoleamine 2,3-dioxygenase 1 (CHEMBL4685)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery and preliminary SARs of keto-indoles as novel indoleamine 2,3-dioxygenase (IDO) inhibitors.
Dolušić E, Larrieu P, Blanc S, Sapunaric F, Pouyez J, Moineaux L, Colette D, Stroobant V, Pilotte L, Colau D, Ferain T, Fraser G, Galleni M, Frère JM, Masereel B, Van den Eynde B, Wouters J, Frédérick R.
Eur J Med Chem (2011) 46 : 3058 -3065
PubMed 21419531 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 1 5.16 5.16
IC50 1 4.04 4.04

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL504816 (L)-1-METHYLTRYPTOPHAN 1 5.16
CHEMBL1802300 1 4.04

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL504816 (L)-1-METHYLTRYPTOPHAN Ki = 7000.0 nM 5.16
CHEMBL1802300 IC50 = 92000.0 nM 4.04