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Assay Detail

CHEMBL1810161

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant GST-tagged VEGFR2 expressed in Sf9 insect cells after 120 mins by Kinase-Glo assay
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type Sf9
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Vascular endothelial growth factor receptor 2 (CHEMBL279)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery and evaluation of 3-phenyl-1H-5-pyrazolylamine-based derivatives as potent, selective and efficacious inhibitors of FMS-like tyrosine kinase-3 (FLT3).
Lin WH, Hsieh SY, Yen SC, Chen CT, Yeh TK, Hsu T, Lu CT, Chen CP, Chen CW, Chou LH, Huang YL, Cheng AH, Chang YI, Tseng YJ, Yen KR, Chao YS, Hsu JT, Jiaang WT.
Bioorg Med Chem (2011) 19 : 4173 -4182
PubMed 21708468 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 6.84 8.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1336 SORAFENIB 4.0 1 8.52
CHEMBL223360 LINIFANIB 3.0 1 8.30
CHEMBL1807469 1 7.16
CHEMBL1807392 1 6.89
CHEMBL1807786 1 6.85
CHEMBL1807471 1 6.80
CHEMBL1807478 1 6.70
CHEMBL1807467 1 6.60
CHEMBL1807483 1 6.54
CHEMBL1807475 1 6.39
CHEMBL1807477 1 6.35
CHEMBL1807480 1 6.25
CHEMBL1807473 1 5.57
CHEMBL1807476 1 -
CHEMBL1807479 1 -
CHEMBL1807484 1 -
CHEMBL1807485 1 -
CHEMBL1807785 1 -
CHEMBL1807474 1 -
CHEMBL1807788 1 -
CHEMBL1807472 1 -
CHEMBL1807470 1 -
CHEMBL1807468 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1336 SORAFENIB IC50 = 3.0 nM 8.52
CHEMBL223360 LINIFANIB IC50 = 5.0 nM 8.30
CHEMBL1807469 IC50 = 70.0 nM 7.16
CHEMBL1807392 IC50 = 130.0 nM 6.89
CHEMBL1807786 IC50 = 140.0 nM 6.85
CHEMBL1807471 IC50 = 160.0 nM 6.80
CHEMBL1807478 IC50 = 200.0 nM 6.70
CHEMBL1807467 IC50 = 250.0 nM 6.60
CHEMBL1807483 IC50 = 290.0 nM 6.54
CHEMBL1807475 IC50 = 410.0 nM 6.39
CHEMBL1807477 IC50 = 450.0 nM 6.35
CHEMBL1807480 IC50 = 560.0 nM 6.25
CHEMBL1807473 IC50 = 2720.0 nM 5.57
CHEMBL1807476 IC50 > 1000.0 nM -
CHEMBL1807479 IC50 > 1000.0 nM -
CHEMBL1807484 IC50 > 1000000.0 nM -
CHEMBL1807485 IC50 > 1000000.0 nM -
CHEMBL1807785 IC50 > 1000000.0 nM -
CHEMBL1807474 IC50 > 1000.0 nM -
CHEMBL1807788 IC50 > 1000000.0 nM -
CHEMBL1807472 IC50 > 1000.0 nM -
CHEMBL1807470 IC50 > 1000.0 nM -
CHEMBL1807468 IC50 > 1000.0 nM -